Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Meridian Life Science Fast qPCR Mix 5x, 40ML
Fast qPCR Mix, 5x has been designed with the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with a fast hot-start polymerase, dNTPs and MgCl2, for highly reproducible, accurate, sensitive qPCR efficiency, even from low abundance targets and scarce samples. With high processivity and robustness, it delivers excellent results in both singleplex and multiplex qPCR assays and confers superior assay performance under fast thermal cycling conditions, allowing more samples to be run in a day with the highest confidence, ideal for high‑throughput assays.
Cayman Chemical Biotoxis qPCR Detection Kit
A qPCR all-in-one detection kit that detects B. anthracis, Y. pestis and F. tularensis.
Meridian Life Science Lyo-Ready qPCR Buffer 2.5x, 8ML
Lyo-Ready qPCR Buffer 2.5x is a glycerol-free qPCR reaction buffer containing lyo-excipients, designed to deliver highly reproducible, accurate qPCR assay results under fast thermal cycling conditions. In order to produce lyophilized, ambient-temperature stable qPCR reagents, dNTP, MgCl2 and glycerol-free Taq DNA polymerase (such as MDX011 or MDX015) must be added prior to lyophilization.
Meridian Life Science RT-qPCR Extraction Control, 1ML
Molecular IVD companies continue to experience tighter and tighter regulations in regard to incorporating appropriate positive and negative controls into their assays. Meridian’s RT-qPCR Extraction Control Orange uses artificial micelles that contain quantitative PCR positive control RNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample target RNA). The cells are added to the RT-qPCR Extraction Control is spiked in the with lysis buffer into the sample prior to RNA extraction for downstream amplification by RT-qPCR. The detection of RT-qPCR Extraction Control acts as a cDNA synthesis and PCR positive control and confirms the success of the extraction and amplification steps, avoiding the misinterpretation of a false negative result.
Meridian Life Science Inhibitor-Tolerant qPCR Mix, 500ML
Meridian’s universal Inhibitor-Tolerant qPCR is a 2x formulation ready-to-use quantitative PCR supermix, containing all components except primers, probes and templates for direct qPCR. It is designed for developing qPCR multiplex assays that require minimal sample processing and fast turn-around times (TAT). Because the mix is highly resistant to various qPCR inhibitors, it can be used with crude lysates or inhibitor-rich samples to directly detect pathogens, including viruses and bacteria, in various biological specimens and to analyze gene expression in cells.
Biochain Institute Inc Hotstart Taq DNA Polymerase, 1000 units/PK
BioChain's HotStart Taq Polymerase combines high specificity, sensitivity, and minimal optimization with a fast 10-minute activation time. During PCR reaction setup, DNA polymerases commonly exhibit some synthesis activity which can lead to amplification of mis-primed sequences and accumulation of primer-dimers that compromise the specificity and yield of the reaction. Non-specific amplification can be minimized with HotStart Taq Polymerase. Our ready-to-use HotStart Taq Polymerase is chemically modified causing its activity to be blocked at lower temperatures. Activity is restored when the enzyme is heated to 95oC during the initial denaturing process, enabling hot-start PCR. BioChain?€?s Hot-Start Taq Polymerase is a powerful, yet cost effective way to minimize pre-cycling polymerase activity for more efficient, specific PCR.
New England Biolabs, Inc. Oligo d(T)23VN – 1 A260 units
Oligo d(T)23 VN is used for the priming and sequencing of mRNA adjacent to the 3'-poly A tail. V = A or G or C N = A or G or C or T
INTACT GENOMICS INC ig SYBR Green qPCR 2X Master Mix 2000 Reactions
Intact Genomics SYBR Green qPCR 2x master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA by qPCR. The Ig SYBR Green qPCR 2x master mix with integrated chemically modified hot start Taq DNA polymerase, SYBR Green I fluorescent dye, ROX dye*, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. This features a high-quality hot start Taq DNA Polymerase for higher fidelity and better amplification.Applications Gene expression validation Absolute quantification Mutation detection Pathogen detection GMO characterization Genetic profilingBenefits Enhanced efficiency, specificity, and sensitivity Compatible with all real-time PCR instruments Superior gene expression results under various cycling conditions Robust and active for cDNA synthesis at temperatures up to 55 °C.
OriGene Villin (VIL1) Human qPCR Template Standard (BC017303) 1 kit
qPCR primer pairs and template standards against Homo sapiens gene VIL1
Eagle Biosciences Inc Human Residual DNA Fragment Analysis Detection KitqPCR
Human Residual DNA Fragment Analysis Detection KitqPCR
Alkali Scientific Radiant™ Probe 1-Step Hi-ROX qPCR Kit, 100 Reactions, (1 X 1mL 2X Probe 1-Step Hi-ROX qPCR Mix, 1 X 0.2mL 20X RTase with RNase inhibitor)
Alkali Scientific offers Radiant™ Probe 1-Step Hi-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Probe 1-Step Hi-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.